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Image Search Results
Journal: Journal of medical virology
Article Title: 25-hydroxycholesterol inhibits human papillomavirus infection in cervical epithelial cells by perturbing cytoskeletal remodeling.
doi: 10.1002/jmv.28834
Figure Lengend Snippet: FIGURE 7 25HC suppresses HPV infection by restricting small GTPase prenylation. (A) Pan‐prenylation level was suppressed by 25HC. (B) Membrane proteins were collected for Western blot. Membrane‐bound, prenylated Rac1, Cdc42, RhoA and Rap1 expressions were reduced by 25HC. Na+/K+ ATPase (ATP1a1) was used as membrane loading control. (C) The prenylation inhibitors (a) GGTI‐298 and (b) FTI‐277 revealed anti‐HPV16 PsV effect in inhibitory assays. GGTI‐298 and FTI‐277 were serially diluted (0−10 μM) in DMEM and incubated with HPV16 PsV. Mixtures were added to cells and incubated for 16 h for inhibition assay. (D) Farnesylation substrate FPP rescued anti‐HPV capability of 25HC. FPP (10 μM) were mixed with serially diluted 25HC, and added to cells with HPV16 PsV. (E) Prenylation inhibitor GGTI‐298 inhibited LIMK1/cofilin phosphorylation.
Article Snippet: Cells were cultured in
Techniques: Infection, Membrane, Western Blot, Control, Incubation, Inhibition, Phospho-proteomics